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In more comprehensive studies, it was showed that, in vitro, the tandem Tudor domain of 53BP1 binds to several dimethylated lysine residues at the p53 C-terminus, with a preference for p53K382me2 172 , 173 The KD value of 53BP1 Tudor-p53K382me2 is about zero.9 µM, whereas that of 53BP1 Tudor-p53K372me2 is 20 µM, indicating that the binding of 53BP1 Tudor to p53K382me2 is way stronger 173 Additional, technology of K382me2 within the p53K370R mutant retains the flexibility of p53 protein to work together with 53BP1 in vivo, suggesting that p53 K382 dimethylation is more seemingly the primary goal of 53BP1 Tudor domains 172 Since other areas of p53 and 53BP1 also work together, additional study is needed to define the specificity and functions of p53 C-terminal lysine methylation for 53BP1 association and different activities.
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A: I live in Lancashire within the North of England with my spouse and two boys.
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